Design and Modeling of Symmetric Three Branch Polymer Planar Optical Power Dividers

Two types of polymer-based three-branch symmetric planar optical power dividers (splitters) were designed, multimode interference (MMI) splitter and triangular shape-spacing splitter. By means of modeling the real structures were simulated as made of Epoxy Novolak Resin on silicon substrate, with silica buffer layer and polymethylmethacrylate as protection cover layer. The design of polymer waveguide structure was done by Beam Propagation Method. After comparing properties of both types of the splitters we have demonstrated that our new polymer based triangular shaped splitter can work simultaneously in broader spectrum, the only condition would be that the waveguides are single-mode guiding. It practically means that, what concerns communication wavelengths, it can on principle simultaneously operate at two mainly used wavelengths, 1310 and 1550 nm

Design of the Novel Wavelength Triplexer Using Multiple Polymer Microring Resonators

We report about new design of wavelength triplexer using multiple polymer optical microring resonators. Triplexer consists of two downstream wavelength channels operating at 1490 ± 10 nm, 1555 ± 10 nm and one upstream wavelength channel operating at 1310 ± 50 nm. The parallel coupled double ring resonator was used for separation of the optical signal band at 1555 nm and filtered out signal bands 1310 nm and 1490 nm. The serially coupled triple optical microring resonator was used for separation of the optical signal band at 1490 nm and filtered out signal bands 1310 nm and 1555 nm. The design was done by using FullWAVETM software by the finite-difference time-domain method. Simulation showed that optical losses for band at 1555 nm were -3 dB and crosstalk between signal bands 1555 nm and 1490 nm was 24 dB. Calculated optical losses for channel 1490 nm were less than -2.5 dB and signal bands at 1555 nm was filtered out with less than 18 dB loss. The bands at 1310 nm were fully filtered out from both downstream wavelength channels operating at bands 1490 nm and 1555 nm

Study of Adjustable Gains for Control of Oscillation Frequency and Oscillation Condition in 3R-2C Oscillator

An idea of adjustable gain in order to obtain controllable features is very useful for design of tuneable oscillators. Several active elements with adjustable properties (current and voltage gain) are discussed in this paper. Three modified oscillator conceptions that are quite simple, directly electronically adjustable, providing independent control of oscillation condition and frequency were designed. Positive and negative aspects of presented method of control are discussed. Expected assumptions of adjustability are verified experimentally on one of the presented solution

Current Gain Controlled CCTA and its Application in Quadrature Oscillator and Direct Frequency Modulator

A modified conception of adjustable current conveyor transconductance amplifier (CCTA) and its interesting application in simple quadrature oscillator expandable for direct frequency modulation purposes, employing only four grounded passive elements is presented in this paper. It is quite simple solution for modern communication subsystem components. An electronic adjusting of the oscillation frequency is easily possible and control of condition of the oscillation is realized via only one grounded resistor. The characteristic equation, condition of oscillation and major parasitic influences of real active part are discussed. The verification includes PSpice simulation and measurement with the CCTA block formed by commercially available active elements

Voltage-Mode All-Pass Filters Using Universal Voltage Conveyor and MOSFET-Based Electronic Resistors

The paper presents two novel realizations of voltage-mode first-order all-pass filters. Both circuits use single universal voltage conveyor (UVC), single capacitor, and two grounded resistors. Using the two NMOS transistors-based realizations of the electronic resistor with two symmetrical power supplies, presented all-pass filter circuits can be easily made electronically tunable. Proposed filter structures provide both inverting and non-inverting outputs at the same configuration simultaneously and they have high-input and low-output impedances that are desired for easy cascading in voltage-mode operations. The nonidealities of the proposed circuits are also analyzed and compared. The theoretical results of both circuits are verified by SPICE simulations using TSMC 0.35 μm CMOS process parameters. Based on the evaluation, the behavior of one of the circuits featuring better performance was also experimentally measured using the UVC-N1C 0520 integrated circuit

Design of Polymer Wavelength Splitter 1310 nm/1550 nm Based on Multimode Interferences

We report about design of 1x2 1310/1550 nm optical wavelength division multiplexer based on polymer waveguides. The polymer splitter was designed by using RSoft software based on beam propagation method. Epoxy novolak resin polymer was used as core waveguides layer, silicon substrate with silica layer was used as buffer layer and polymethylmethacrylate was used as protection cover layer. The simulation shows that the output energy for the fundamental mode is 67.1 % for 1310 nm and 67.8 % for 1550 nm wavelength

Design, Fabrication and Properties of Rib Poly(methylmethacrylimide) Optical Waveguides

We report about design, fabrication and properties of the polymer optical waveguides deposited on silica-on-silicon substrate. The design of the waveguides is based on a concept that geometric dimensions of the single mode polymer waveguide are determined by geometrical parameters of the silica layer. The design of the waveguides was schemed for 650 nm, 850 nm, 1310 nm and 1550 nm wavelength. The design of the presented planar waveguides was realized on the bases of modified dispersion equation while the ridge waveguides design was proposed following the Fischbeck concept. Both designs were refined applying RSoft software using beam propagation method. Proposed shapes of the waveguides were etched by standard photolithography process into the silica layers and polymer waveguide layers were subsequently deposited into the treated substrate by spin coating. Poly(methylmethacrylimide) was used as the waveguide core material and polymethylmethacrylate was used as a cover protection layer. Propagation optical loss measurements were done by using the cut-back method and the best samples had optical losses lower than 0.6 dB/cm at 650 nm, 1310 nm and 1550 nm

Designer lipid-like peptides

A crucial bottleneck in membrane protein studies, particularly G-protein coupled receptors, is the notorious difficulty of finding an optimal detergent that can solubilize them and maintain their stability and function. Here we report rapid production of 12 unique mammalian olfactory receptors using short designer lipid-like peptides as detergents. The peptides were able to solubilize and stabilize each receptor. Circular dichroism showed that the purified olfactory receptors had alpha-helical secondary structures. Microscale thermophoresis suggested that the receptors were functional and bound their odorants. Blot intensity measurements indicated that milligram quantities of each olfactory receptor could be produced with at least one peptide detergent. The peptide detergents' capability was comparable to that of the detergent Brij-35. The ability of 10 peptide detergents to functionally solubilize 12 olfactory receptors demonstrates their usefulness as a new class of detergents for olfactory receptors, and possibly other G-protein coupled receptors and membrane proteins

Rapid dissemination of Francisella tularensis and the effect of route of infection

<p>Abstract</p> <p>Background</p> <p><it>Francisella tularensis </it>subsp. <it>tularensis </it>is classified as a Category A bioweapon that is capable of establishing a lethal infection in humans upon inhalation of very few organisms. However, the virulence mechanisms of this organism are not well characterized. <it>Francisella tularensis </it>subsp. <it>novicida</it>, which is an equally virulent subspecies in mice, was used in concert with a microPET scanner to better understand its temporal dissemination in vivo upon intranasal infection and how such dissemination compares with other routes of infection. Adult mice were inoculated intranasally with <it>F. tularensis </it>subsp. <it>novicida </it>radiolabeled with ⁶⁴Cu and imaged by microPET at 0.25, 2 and 20 hours post-infection.</p> <p>Results</p> <p>⁶⁴Cu labeled <it>F. tularensis </it>subsp. <it>novicida </it>administered intranasally or intratracheally were visualized in the respiratory tract and stomach at 0.25 hours post infection. By 20 hours, there was significant tropism to the lung compared with other tissues. In contrast, the images of radiolabeled <it>F. tularensis </it>subsp. <it>novicida </it>when administered intragastrically, intradermally, intraperitoneally and intravenouslly were more generally limited to the gastrointestinal system, site of inoculation, liver and spleen respectively. MicroPET images correlated with the biodistribution of isotope and bacterial burdens in analyzed tissues.</p> <p>Conclusion</p> <p>Our findings suggest that Francisella has a differential tissue tropism depending on the route of entry and that the virulence of Francisella by the pulmonary route is associated with a rapid bacteremia and an early preferential tropism to the lung. In addition, the use of the microPET device allowed us to identify the cecum as a novel site of colonization of <it>Francisella tularensis </it>subsp. <it>novicida </it>in mice.</p

A Robust and Rapid Method of Producing Soluble, Stable, and Functional G-Protein Coupled Receptors

Membrane proteins, particularly G-protein coupled receptors (GPCRs), are notoriously difficult to express. Using commercial E.coli cell-free systems with the detergent Brij-35, we could rapidly produce milligram quantities of 13 unique GPCRs. Immunoaffinity purification yielded receptors at >90% purity. Secondary structure analysis using circular dichroism indicated that the purified receptors were properly folded. Microscale thermophoresis, a novel label-free and surface-free detection technique that uses thermal gradients, showed that these receptors bound their ligands. The secondary structure and ligand-binding results from cell-free produced proteins were comparable to those expressed and purified from HEK293 cells. Our study demonstrates that cell-free protein production using commercially available kits and optimal detergents is a robust technology that can be used to produce sufficient GPCRs for biochemical, structural, and functional analyses. This robust and simple method may further stimulate others to study the structure and function of membrane proteins.United States. Defense Advanced Research Projects Agency (DARPA-HR0011-09-C-0012)Massachusetts Institute of Technology. Undergraduate Research Opportunities Progra